Dr. MAHA Saeed Ibrahim WIZRAH
علم الخليه والاحياء الجزيئيه Arts And Sciences in Wadi Addawasir - PhysicsOverview
Dr. MAHA Saeed Ibrahim WIZRAH Faculty member in the field of علم الخليه والاحياء الجزيئيه College Arts And Sciences in Wadi Addawasir At Prince Sattam bin Abdulaziz University, he contributes to supporting the educational and learning process by offering academic courses, participating in educational activities and programs, and supporting the development of students' skills and enhancing their knowledge in the field. علم الخليه والاحياء الجزيئيه
Academic qualifications
Academic progression
Research interests
• In silico construct design for protein expression in Escherichia coli and genetic manipulation in Cryptococcus neoformans
• Molecular cloning techniques (PCR, NEBuilder assembly, digestion with restriction enzymes, bacterial transformation, cracking buffer to screen for colonies with positive inserts, verifying sequence errors by Sanger sequencing)
• Native protein expression and purification in C. neoformans via HA antibody coated beads
• Recombinant protein expression via autoinduction in E. coli and purification using immobilized metal affinity chromatography (His-column) and size exclusion chromatography (S200 column attached to a ÄKTA™pure FPLC system)
• Oligomeric state analysis via mass photometry using Refeyn OneMP device
• Biochemical enzyme kinetic characterization continuous assays using UV-Vis Cary60 spectrophotometer
• Phenotype testing by spotting dilutions of fungal strains
• Mice handling, infection of mice through inhalation, mice euthanasia by CO2, dissection and collection of mice organs, processing and spotting homogenized organs on agar media
• Protein crystallization, data processing and solving crystal structure
• Flow cytometry for measuring fluorescence using CytoFLEX flow cytometer
• Proficient in many computer programs:
• Microsoft Office, Microsoft Excel, Microsoft Powerpoint and Endnote referencing software
• Adobe illustrator for publication quality figure creation
• MacVector for designing genetic constructs
• Chemdraw, Prism and ImageJ for data visualization and analysis
• CLC genomics workbench for analyzing Sanger sequencing data
• Crystallography software: Coot, PyMOL, CCP4, Phenix
Published research
View all research (10)Courses
9 Decisions| Course code | Course Name |
|---|---|
| BIO 2502 | Microbiology |
| APPS 1313 | Applied Training |
| CASE 2611 | Case Study |
| CHEM 2504 | Polymer Chemistry |
| BIO 106 | General Biology |
| CT 2401 | Quality Management in Laboratories |
| BIO 1202 | General Biology |
| APPS 2610 | Applied training |
| CT 1201 | Safety and Security in Llaboratories |
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